What the Experts Are Saying About Mental Health Argumentative Essay Topics and How It Affects You

What the Experts Are Saying About Mental Health Argumentative Essay Topics and How It Affects You Argumentative essay topics are so important since they are debatableand it's important to at all times be critically considering the world around us. Moral argumentative essay topics are a few of the simplest to get carried away with. Choosing topics for argumentative essays is critical for your general success. Recent argumentative essay topics that are related to society is going to do. Examine the list to choose the topic which can help you compose a creative essay for your middle school class. The intent of assigning an essay to middle school students is to make awareness and permit them to develop writing skills. With proper main topics, they can reach good results. At various classes, they learn how to perform great articles. What You Must Know About Mental Health Argumentative Essay Topics It's possible to restate your argument, which is a rather common practice amongs t essayists. 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Relevant Topics that are related to your society will engage the reader in the most effective possible method. English language c lasses usually take a lot of writing. F. Bacon The main goal of the capital isn't to get as much money as possible, yet to guarantee that money produces a better life. New Ideas Into Mental Health Argumentative Essay Topics Never Before Revealed Argumentative essays are a few of the best that you can write as a student. All persuasive essays are like argumentative essays. Easy expository essays are essays where you're anticipated to work on facts instead of opinions. An argumentative essay is a certain sort of academic writing. You are able to download our sample essay papers free of charge. There are several steps which you should take as a way to compose a superb essay. You don't need to find super technical with legal argumentative essays, but remember to do your homework on what the present laws about your preferred topic actually say. When you have followed all of the above steps, you'll be in a position to produce an extremely good argumentative essay, which could be written in MLA format or APA format. Since you may see, there's a plethora of different argumentative paper titles you may utilize. Every student who would like to perform an ideal academic paper can use mentioned subjects and example argumentative essay to do an exceptional work. It is extremely important to select a great topic so as to compose a terrific paper. It's possible to opt for an intriguing topic from any area of science. What's Truly Going on with Mental Health Argumentative Essay Topics Deciding on your topic isn't that easy. There are plenty of approaches that could help you to select a topic. Quite frequently, the ideal topic is one which you truly care about, but you also will need to get ready to research it. Selecting an emotional topic is also an excellent idea. The second thing which you should take care of once you choose the title is to maintain in mind five varieties of argument claims. Some folks live their lives based on their religion while some don't think you should factor that into decision making in regards to determining rules for everybody. When you're picking your topic, bear in mind that it's much simpler to write about something which you currently have interest ineven in case you don't know a great deal about it. Canadian students must deal with precisely the same problem of selecting engaging argumentative essay topics as the remainder of the world.

The Secret Language Of Clinical Psychology - 972 Words

The Secret Language of Clinical Psychologists. The article, Women Entering Clinical Psychology: Q-Sort Narratives of Career Attraction of Female Clinical Psychology Trainees in the UK was published on September nineteenth, 2011 by Martyn Baker and Jen Nash. This article discusses about a vast amount of women in the UK are attracted to the clinical psychology workforce. This article is on a study that tests how the women are attracted to clinical psychology through the use of five narratives of attraction. This article can be very difficult to understand if the reader is not part of the clinical psychology discourse community. In order to understand this article, one would need to know how to read Psychology journal articles. Psychology journal articles are made up of five different sections, that is; the abstract, introduction, method section, the results section, the discussion section, and the references section. In this article, the abstract and introduction summarized what Baker and Nash are looking for and how they got their answers. The method section and results section shows the way in which the research came about and what the procedures used and variables measured are and what were the results through the use of tables and figures to explain exactly what they mean. The discussion section is a summary of the entire article and what the tables and figures mean. Lastly, the references section shows all the cited articles and sources used in this article. The audienceShow MoreRelatedClinical Psychology : Mental Illness, Emotional, And Behavioral Disorders1027 Words   |  5 PagesClinical Psychology is a branch of psychology that work with treating mental illness, emotional, and behavioral disorders. Psychologists work with patients that have disabilities such as depression, anxiety, eating disorders and substance abuse. 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Police Brutality And Violence On Black People - 1479 Words

I am an educated black man, business owner, father, husband, I apologize I look like a thug. This is a quote from one of the signs held up at a protest for Michael Brown. Michael Brown was shot by a police officer named Darren Wilson. Brown was shot about six times after Wilson targeted him for walking in the street with a friend. Police brutality and violence on black people has become prominent and indicates racism, negative stereotyping, and white supremacy in full effect with the police. Michael Brown is one of many crimes done be the real criminals in uniform. To begin, racism, which is a social construction is no doubt embedded in America s police and government. It is an epidemic that has gone on for centuries where white people are dominant and superior to other races, particularly black people. Racism has structured the lives of black people to be inferior and harassed by authorities because many whites view blacks as a threat. The only way for white people to eliminate any fears and negative stereotypes about black people is through the police targeting them. In the article â€Å"What is Racial Domination?† it states, Because racism infuses all of social life, people of color and whites alike develop thoughts and practices molded by racism; people of color and Whites alike develop stereotypes about racial groups (Desmond Emirbayer, 2009:347). This statement explains that black people and white people both do develop stereotypes about other and even their ownShow MoreRelatedA Raisin In The Sun Is Play Written About A Family In The1592 Words   |  7 Pagesdeal with discriminatory housing practices and the threat of racial violence. A Raisin in the Sun is relevant today because a lot of the insights it makes about racism are still debated over today. One of the issues it tackles is racial violence. 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In the past, police officers were considered peacekeepers. Police have been occupied in shootings, severe beatings, and unnecessarily rough treatment s to our people instead of focusingRead MorePolice Violence Has Gone On Since Slavery Times959 Words   |  4 PagesPolice violence has gone on since slavery times, but had a big impact on the early 40’s- late 60’s. On June 27, 1969 Helen Smith stood with Donald Best, patrolman Alvin Nachman approached with his dog and ordered to â€Å"hold the noise down†. No neighbors had complained about anything, the police dog attacked Ms Smith and as she fought off the officer maced Ms Smith. She had to get a total of 75 stitches over her body, and was charged with assault on an officer (Alexandria Wicle). In the 90’s police

The Whiskey Rebellion Essay Example For Students

The Whiskey Rebellion Essay Mike Klenosky11/24/96AP History per. 4Ms. ValentinoOn August 1, 1794, President George Washington was once again leadingtroops. Only this time Washington was not striking out against the British butrather against fellow Americans. The occasion for this was the Whiskey Rebellion. Various efforts had been made to diminish the heated opposition towards the taxon distilled liquors. However, there was only one man who has derived the bestcourse of action. That man, President George Washington, deserves all the creditand recognition for his actions concerning the Whiskey Rebellion. In September 1791 the western counties of Pennsylvania broke out inrebellion against a federal excise tax on the distillation of liquor. Afterlocal and federal officials were attacked, President Washington and his advisorsdecided to send troops to assuage the region. On August 14, 1792, under themilitia law, Henry Knox (secretary of war) had called for 12,950 troops. Afterthis, many problems arose, both political and logistical. These dilemmas had tobe overcome, and by October, 1794 the men were on the march towards Harrisburg,Pennsylvania. There, they contained the mob hysteria and anger. This eventrepresented the first use of the Militia Law of 1792 enabling the militia to execute the laws of the union, and suppress insurrection (The Whiskey Rebellionof Harrisburg, Pennsylvania, 1). It is clear that George Washington was the source of success in theWhiskey Rebellion. When the militia, with Washington and Hamilton at its lead,reached western Pennsylvania, it became clear that there would be no armedresistance. Evidence of Washingtons leadership in this rebellion took placewhen the Representatives of the insurgents asked for clemency, and Washingtongranted it with stipulation that they comply with federal laws thereafter (ThePrecipice of Power). This agreement forced the public to abide by the rules ofthe government and their taxes without any destructive rebellions. It wasevident that Alexander Hamilton was not the backbone of this success. Hisactions provided undeniable proof to Republicans that Hamilton was a monster whowould stop at nothing to defend his corrupt policies, a budding Caesar bent onestablishing monarchy (A Biography of Alexander Hamilton). Hamilton did notcare as much about the success of his government but of himself and his beliefson the nation . Furthermore, Hamilton was planning on resigning, hence making itcrucial to him to entrench the policies he had put into place. For theremainder of his life Hamilton worried that his work would be destroyed, hissystem dismantled, under the opposition (The Precipice of Power). President George Washington played a key role in the opposition betweenthe mob and the militia. He deserves the credit for creating and maintainingpeace among the people, and carrying out the mission without one shot fired. Hamilton, on the other hand, put his interests ahead of the problem at task,hence, forcing Washington to come up with a logical solution. Had it not beenfor Washingtons courage and kindness, the militia might well have followed thelead of the French Rebels, and destroyed the country.

The 2012 London Olympic Games Human Resource Management

Introduction The 2012 London Olympic games is an international event, which not only brings the world together, but it also reflects a nation’s coeffective synchronization throughout its planning and successful realization of its objectives and the courage to sustain and harmonize all groups associated with the event.Advertising We will write a custom report sample on The 2012 London Olympic Games Human Resource Management specifically for you for only $16.05 $11/page Learn More It is also an event that has rapidly developed a considerable contribution to businesses and other leisure related activities that integrates all forms of tourism at an extensive level. The magnitude of the event comes with the increased growth of both government and corporate involvement, which further complicates the environment that now calls for a high level of resonance from the Olympic committee and event managers who should identify and tune-up with a wider range of stakeholders in order to bring a sense of balance to their needs and main objectives (Iryna 2009, p.10). For this reason, Human Resource Management (HRM) in any goal oriented organization should be deeply concerned with how to secure and coordinate the various kinds of resources that takes account of facilities, materials, finances, and people, who are the most critical yet the most problematic group since they represent the human resource. The environment that surrounds the preparation for the Olympic event is such a challenging task since it deals with a handful of both logistical concerns and a diverse team of individuals, and for this reason, the HRM process is an effective way of designing and staging the event successfully by giving a critical look on the overall Olympic context and learning from past mistakes witnessed in other past events of the same magnitude. Since the Olympic games is a long-awaited event and a highly rated event, it’s always advisable to carefull y note the importance of personnel management through the administrative functions it encompasses in both customary and traditional means that work towards bringing employee motivation and satisfaction with things such as rewards, bonuses, compensation, and the overview of work responsibilities. For this reason, HRM practices are profusely merged with personnel administration to form work groups and effective strategies that will address any challenges that may arise in the course of event planning and job creation which are just some of the primary motivators leading up to the event (Chelladurai Alberto 2006 p. 65).Advertising Looking for report on business economics? Let's see if we can help you! Get your first paper with 15% OFF Learn More An effective HRM practice should is expected to consequently recruit, orient and train good employees who are either employed casually or temporarily, in order to acquaint them with the necessary objectives and targets that call for the adequate use of time and resources (Wilton 2010, p.165). The changing alignments in HRM strategy and internal practices in the modernized world is more perceptible when effectively implementing business strategy since the event also aims at making profit, while showcasing brilliance in its management planning. A special consideration should therefore be given to the people, who are usually the ones who convert all the other factors of the Olympic process into ‘real’ resources that can be worked upon. Therefore, a careful way of dealing with the workforce should be very cognizant of their rights, needs and values (Lynn, 2006, p.16). For instance, equipments and facilities such as a computer, basketball, and an ice rink are only resourceful when they are competently used for their purpose in an appropriate manner. Therefore, from this perspective, the 2012 London Olympic Games Committee should combine numerous features that are universally shared with other nonpr ofit associations that have inclusive characteristics of the typical sporting sector in the broadest manner, while also deriving values and main cultural features that specifically correlate the Olympic movement and spirit. The HRM practice for the Olympic Games should be highly prioritized since it is a package of a wide range of products that includes goods and services, which are primary to the event that should also incorporate an elevated consideration into transport, service, food, queues, tickets, the environment, security, and the event merchandises. In other words, the role of strategic HRM is highly emphasized and the importance of its fitting with the external environment (Chelladurai Alberto 2006, p 31-65). Therefore, the general event product should be an inimitable blend of activities which are the operational tools for maximum achievement of the much sought after satisfaction, needs, and aims of the Olympic event upon its instigation and conclusion. The 2012 London O lympic event must then meet all the necessary human needs at all levels which is in concert with the management of human resources. By doing this, the HRM practices will realize a critical process of product planning for its main objectives, while also providing an optimal experience to the audience and the world at large.Advertising We will write a custom report sample on The 2012 London Olympic Games Human Resource Management specifically for you for only $16.05 $11/page Learn More As an independent Human Resource Consultant, the following outlooks will help guide the UK Olympic committee for the 2012 games on some fundamental concerns and proposals that will help forge unity among the team that comprises thousands of casual and temporary staff. The Human Resource Planning and Recruitment Process The main purpose of the Olympic committee is to formally recognize the main purpose of the event while also monitoring the progress towards realizing these g oals, and the committee members should be responsible on matters dealing with finance, marketing, production, operations, and staffing. The HRM process portrays much more than just the selection an d recruitment of staff and volunteers and the Olympic committee should therefore select a highly competent in-line managers and directors who posses an exemplary experience in such assignments so that they will constantly be their hands, eyes, and ears on the ground in order to oversee the foundation of the overall project while effectives dealing with the personnel who are most instrumental at this stage(Chelladurai Alberto, 2006, p. 31-65). The Human Resource (HR) managers should be tasked with the authority to direct the activities of the people in their departments and any other service areas that are interrelated. Although the authority they posses come with the proficiency of their job description within the HR department, they should not over-exert their implied authority and cruc ial decisions should only be left with the committee members. Each departmental manager should also synchronize personnel activities for the purpose of a functional authority which will ensure that the HR policies and practices are effectively implemented. However, in order to meet the following obligations regarding unity enforcement, the Olympic committee should include various stakeholders and social partners to sequentially address matters dealing with their efficiency and cost-effectiveness. Design and implementation of a staffing policy This policy should encourage equality in the staff hiring process and will emphasize on a thorough examination of job candidates and considerably adhering to their academic or skill qualifications before they are hired. This eliminates nepotism and other forms of corruption associated with the process and significantly promote a diverse pool of job applications. The potential candidates are also required to undertake various personal tests that will confirm their suitability. This brings in professionalism, efficiency, and credibility within the organizations as only the best will be considered. It will also help the process stick to its ethics by eliminating unacceptable interview questions.Advertising Looking for report on business economics? Let's see if we can help you! Get your first paper with 15% OFF Learn More However, this policy can meet its full potential if the hiring decisions take into account the level of degree to which the candidates demonstrate through their past actions the values vital to the project’s philosophy that encourages diverse workplace relationship (Burke Copper 2005, p. 161). This is process stands out to be most fundamental since it will put a pervasive mood on the whole event which is likely to be identified with both local and international stakeholders. This process will ensure that the most skilful and well capable persons are chosen without adhering to the various dimensions of discrimination that may range from race, ethnicity, age, culture, social status, sexual orientation, ethnicity, religion, language, and any other form of preconceived notion. The selection process should not cut off any persons willing to volunteer on the event project and should act on bringing diverse groups together since its one of the main objectives of the whole event. Th e hiring process should also be well structured and convenient for optimal interview training systems with an interview panel overseeing the recruit process with appropriate job duty that is relevant with ones profession and qualifications (Wilton 2010, p. 153). In general, diversity in this process is a first step towards creating an inclusive work environment that will eventually motivate employees, improve the interpersonal skills of staff members, while encouraging creativity and comprehensive innovation. In order to foster local unity that will eventually pass onto the staff, the recruitment process should also engage the local community and organizations together with other selection methods that will give consider the On Demand Recruiting Services (ODRS) since it’s a specialized recruiting service that works to support short-term projects with no expense of keeping hold of traditional search firms. Due to the fact that the Olympic event project works on a specific time line, such recruit services will help in the expenditure planning since they are paid by hour or even on the project worked as an alternative to the percentage fee. The general concept in this process is to manage diversity that will help promote the appreciation of service, thoughts, and understanding which will help unify our employee differences that can eventually turn into an asset with work being done cooperatively, effectively, and more efficiently. Design and implement an anti-harassment policy that protects Diversity Group Members Diverse groups facing any form of harassment and victimization are legally protected in the law and the violation of this law is grounds for punitive action by any disciplinary body. Such claims should first be channeled through departmental heads who should try solving the issue internally. This will help in combating any hate speech or action against minority members. It will motivate competent and talented members join a workplace knowing that they will be judged on merit and not stereotypes (Wilton 2010, p.165). It will also set in harmony in the workplace and improve productivity as no time will be wasted on unwarranted squabbles. However, this policy should be generalized in the workplace so that everyone is included, and by doing this no one is exceptional and can be prosecuted or have his contract being terminated. This will gradually change mindsets and attitudes between employees and set in respect. Design and implement a global equity policy This policy will help to provide all indispensable ingredients needed in the efforts of reducing gender inequalities among the event staff. It will also improve productivity in large through a balanced and fair workplace relations system, in consistent with the organization’s core values. It will provide for equal remuneration of work and its value, and protect genuine workplace representation while enshrining collectiveness. A global employment equity and representatio n in the workplace will make a vast difference in the social and economic fate of many around the world, and as well as improving competitiveness among organizational enterprises. In order for this policy to meet its obligations, organizations should raise staff awareness by designing and conveying programmes that support equity aims (Chelladurai Alberto 2006, p.65). Train Managers on the requirements of the Employment Equity Act This creates a cordial working relationship between the manager and employee because diverse personnel comprise a massive set of values, beliefs, understandings, unique information, and ways of viewing the world. Managers will know the rules and regulations needed when working with a diverse workforce, and this will help in elimination of cases associated with rights infringement. This will also help in solving any grievances within the workplace. It will also help eliminate unfair promotion, training, suspension, test, or demotion of an employee by the ma nagers. Generally it will eliminate stereotype mind-sets at managerial level. However, this fairness in the workplace can be promoted through workshops that will help managers see a positive side of an employee through communication. Managers should openly exhibit and champion diversity in the workplace by investing more time and effort in recognizing and inviting diverse backgrounds into the Olympic project, and also holding out social events that will foster this understanding (Epstein Manzoni 2006, p.222). Implement and Utilize Information Technology to Promote Unity The introduction of Information Technology (IT) in HRM has over the years had a tremendous significance for both the management-employee cooperation and communication. The application of IT in the Olympic project can also be used in a wide range of choices to include alternative options within the work organization. The management should then required to enforce these changes that will make way of the use of technol ogical equipments and facilities that will help foster communication for all associated with the Olympic event(August Jennifer 2004, p.322). However, in order to bring understanding, the management should train and negotiate with its employees and their representatives to sequentially make relevant changes while ensuring that these arrangements will effectively bring proficiency to those working with IT (Desleigh, Marcia Sylvia 2006, p.15). Therefore, by setting up a communication centre, employees are able to communicate with the management and vice versa. The general public can also benefit from this since they can also get any relevant assistance regarding the progress of the Olympic project. Both the HRM system and employees in the 2012 London Olympic project can all utilize various Wireless Communication equipments, and back-to-back digital interconnectivity systems and equipments that rarely requires anything complicated than the standard exchange of e-mails, making phone ca lls to colleagues, and coordinating meeting times since such a global event incorporates individuals from different time zones (August Jennifer 2004, p.322). These technological equipments can also help both management and employees in the coordination of day-day work, while remotely supporting the numerous business activities within the project. As a result, each managerial department should be suitably quipped with communication equipments that will promote the free-flow of work and communication within such a wide personnel network. Incase of an urgent notification or meeting, both the management and staff can remotely communicate through teleconferencing and videoconferencing systems if the situation does not call for a personalized meeting. These equipments will also harmonize coordination among staff of different departments in regards to project budgeting, marketing, sales, ands so on. Since the deployment of IT equipments stands a good chance of eradicating any form of conf usion between employees, each staff member in various departments should be provided with communications equipment in order to maximize their potentiality in the project. The IT form of communication will help eradicate and any type of paper communication that is unreliable. It will also help locate and account for employees who are on site or anywhere else incase of an emergency situation through phone call confirmation or even location them through GPS system. Conclusion These policy procedures are meant to improve the overall HRM level of standards since it’s a major component of any organizational setting that strives to attain a certain objective. The Olympic event is heavily swayed by the current social and environmental trends which have dire consequences if ignored. In this case, a comprehensive HRM system should aim at addressing financial, logistical, and Staff concerns. The prevalent issues concerning employee disunity sets upon stereotypes due to a non-diversified work environment, and the 2012 London games is one event which should detest such claims by promoting unity amongst staff. The increasingly globalized world also calls for a diverse workforce or else, such an event would competitively ‘get beaten’ in the market place, encompass low productivity rates or face image risk. As an instrument against discrimination, organizational diversity can also be viewed as a strategic resource in eliminating this social-vice. Unity can also be promoted effective if employees are duly compensated for an outstanding job done and should not be selective in awarding them. However, some disunity also does arise due to a shortfall in communication and the use of technology can prove to be a tremendous achievement in the overall London Olympic Games. Bibliography Burke, J. R. Copper, L. C. 2005, Reinventing Human Resource Management challenges and new directions, Routledge, New York. p. 161-162. Chelladurai, P. Alberto, M. 2006, Human resou rce management in Olympic sport organizations, Volume 3, Human Kinetics Publishers, Illinois. p. 31-65. Desleigh, D. J., Marcia, J. S. Sylvia, R. 2006. Assistive technology in the workplace. Elsevier Health Sciences. Philadelphia. P 13-15. Epstein, J. M., Manzoni, F. J. 2006, Performance measurement and management control: improving organizations and society, Emerald Group Publishing. West Yorkshire, England. P 214 – 222. Iryna, S. 2009, Managing Diversity in the Workplace,GRIN Verlag, Norderstedt, Germany. Lynn, W. 2006. Human Resource Management for Events: Managing the Event Workforce, Elsevier, Massachusetts. P 14-16. August, E. G. Jennifer, H. M. 2004, Communication Technology Update, Focal Press Massachusetts. P 321-322. Wilton, N. 2010. An Introduction to Human Resource Management, SAGE Publications California. Pp.153 -165. This report on The 2012 London Olympic Games Human Resource Management was written and submitted by user Sarah Hooper to help you with your own studies. You are free to use it for research and reference purposes in order to write your own paper; however, you must cite it accordingly. You can donate your paper here.

Free Essays on Somalia

Introduction This paper will discuss the Somali social and political organizational structure which is based on kinship groups. There are six main clan- families, the Dir, the Darod, the Isaq, the Hawiye, the Digil and the Rahanweyn. These clans-families are further subdivided into smaller clans. These clans, in turn, are subdivided into sub clans, or primary lineage groups. It is also claimed that all Somalis trace their origin t two brothers, Samaal and Saab (Sab). These brothers are said to have been members of the Prophet Muhammad’s tribe, the Qurash of Arabia. Although his can not be proven, most Somalis know this to be true. (Fox, pg 31) History Somalia is the easternmost country on the African continent. Its capital and largest city is Mogadishu. Somalia is often referred to as the Horn of Africa. Almost all of the Somali people are Sunni Muslims and speak the Somali language. However, some Somalis do speak Italian, Arabic, and English. The official name for Somalia is the Somali Democratic Republic. It became an independent nation in 1960. Before that Somalia’s northern section was ruled by the British and the Southern section was ruled by Italy. Most of Somalia came under colonial rule during the 1800’s. During the mid 1880’s the British occupied much of northern Somalia and they named it British Somaliland. In the late 1880’s, the Italians gained control over the southern part of Somalia and established the colony of the Italian Somaliland. By the 1900’s Somali nationalist began to fight the British Italians and the Ethiopians to regain control of their land. During this crucial time in Somalia history, Sayyid Muhammad Adbille Hassan was the leader of the nationalist and was destined to become a great leader. He was born on April 7, 1864 and was a member of the Ogaden Clan. â€Å"At the early age of seven, Muhammad began to learn the Quran under a local teacher, and by the age of ten, when his grand... Free Essays on Somalia Free Essays on Somalia Introduction This paper will discuss the Somali social and political organizational structure which is based on kinship groups. There are six main clan- families, the Dir, the Darod, the Isaq, the Hawiye, the Digil and the Rahanweyn. These clans-families are further subdivided into smaller clans. These clans, in turn, are subdivided into sub clans, or primary lineage groups. It is also claimed that all Somalis trace their origin t two brothers, Samaal and Saab (Sab). These brothers are said to have been members of the Prophet Muhammad’s tribe, the Qurash of Arabia. Although his can not be proven, most Somalis know this to be true. (Fox, pg 31) History Somalia is the easternmost country on the African continent. Its capital and largest city is Mogadishu. Somalia is often referred to as the Horn of Africa. Almost all of the Somali people are Sunni Muslims and speak the Somali language. However, some Somalis do speak Italian, Arabic, and English. The official name for Somalia is the Somali Democratic Republic. It became an independent nation in 1960. Before that Somalia’s northern section was ruled by the British and the Southern section was ruled by Italy. Most of Somalia came under colonial rule during the 1800’s. During the mid 1880’s the British occupied much of northern Somalia and they named it British Somaliland. In the late 1880’s, the Italians gained control over the southern part of Somalia and established the colony of the Italian Somaliland. By the 1900’s Somali nationalist began to fight the British Italians and the Ethiopians to regain control of their land. During this crucial time in Somalia history, Sayyid Muhammad Adbille Hassan was the leader of the nationalist and was destined to become a great leader. He was born on April 7, 1864 and was a member of the Ogaden Clan. â€Å"At the early age of seven, Muhammad began to learn the Quran under a local teacher, and by the age of ten, when his grand...

Transforming growth factor-beta and ROCK inhibitor in immunostaining Lab Report

Transforming growth factor-beta and ROCK inhibitor in immunostaining and microscopic analysis of adherent cells - Lab Report Example This report examined the transforming growth factor-beta and ROCK inhibitor in immunostaining and microscopic analysis of adherent cells. For a high gene dosage to be achieved, the heterologous cDNAs are normally cloned into replicate plasmids in a fashion that is relaxed and are normally existence at 15-60 copies per cell (Baneyx, 2009). Whenever the additional co-overexpression gene product is needed, ColE1 derivatives are normally put together with plasmids that are compatible and a p15A replication that is maintained at approximately 10 to 12 copies per cell. Under conditions of the laboratory, the multicopy plasmids will be distributed randomly during the division of the cell, and whenever selective pressure are lost at a frequency that is low. This may be due to multimerization (Baneyx, 2009). Whenever there is a high number of copy plasmids, the loss in plasmid can increase tremendously especially when the plasmid bone genes are toxic towards the host or significantly reduce t he rate of growth whenever cells are cultivated at densities that are high or in processes that are continuous. These problems can be addressed by using the encoded plasmid antibiotic markers resistance and the supplement growth medium supplemented to do away with the free cells of the plasmid. One key limitation of this approach involves the loss of selective pressure due to the degradation of antibiotics, leakages, or inactivation of the periplasmic detoxifying enzymes into the medium growth and the product contamination (Baneyx, 2009). This drawback could be unacceptable from a regulatory or medical point of view. In this respect, many alternative methods have been established to make sure that the cells that are free from plasmid will not overtake the culture. This means that cloning vectors will be engineered to carry repressors or genes, which leads to cell death whenever there is a loss of plasmid (Cregg, 2000). Even though, this method is proved to be vital, it could place r estrictions on the medium growth composition whenever there exist any complication and may introduce a burden metabolism on the cell through requiring transcriptions and translation of additional genes of encoded plasmids. For these problems to be circumnavigated, research has established a host strain having a conditionally essential gene in control of the promoter-operator region and a multicopy companion of the plasmid having the lac operator (Baneyx, 2009). Whenever the LacI receptor protein is titrated by encoded plasmid lac operators, it leads to the gene chromosomal expression and plasmid growth that is selective. It may also bear cells in the medium that is supplemented by the antibiotics. Another different solution to the plasmid instability problem could be a direct insertion of the genes that are heterologous within the E coli chromosomes. Even though, there exist a single vehicle delivery like the bacteriophage in this purpose there has been extremely little emphasis pla ced on the perceived notion that the dosage of the gene will always be low. In order to gain more insight on the characteristics of the E coli, this experiment was set to investigate the recombinant protein expression. Methodology. Material. The materials used for this experiment include: EcoRi/HindIII cleaned and cut pUC19 vector (V), EcoRi/HindIII cle